Genome-wide Prediction of Coactivator-controlled Transcriptional Networks Using Data from Ultrahigh-throughput Sequencing Technologies
In all tissues having a high oxidative capacity, and particularly in skeletal muscle, the peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1alpha) powerfully activates mitochondrial biogenesis and aerobic respiration. Although some genes have already been shown to be significantly induced by PGC-1alpha, the complete set of primary targets controlled by this highly versatile protein and the core set of transcription factors (TFs) recruited to their regulatory sites still needs to be defined. Taking advantage of next-generation deep sequencing technologies, expression arrays and other complementary wet-lab techniques, we apply computational methods for integrating these different sets of high-throughput data and, thus, to dissect the transcriptional network driven by PGC-1alpha.
Keywords: Transcriptional Regulation, PGC-1alpha, Coactivator, ChIP-seq, Skeletal Muscleback